![]() Some recipes - like Martin's Bagels or Jeffrey's Bagels with Pâte Fermentée - also add a preferment, which increases the overall fermentation time. For this reason, most good bagels get a long, cold proof to develop flavor. How you ferment a bagel also determines its taste. The longer the shaped bagels sit before baking, the more tender and open-crumbed they’ll tend to be, since the tension built into them during shaping will have had time to relax. Other recipes have you shape the dough into bagels and then proof them for a lengthy stretch, usually in the fridge. Some recipes ferment the dough in bulk (as one large batch of dough before dividing) and then shape, boil, and bake the bagels in quick succession. The next element that goes into a bagel’s texture is how it's fermented. You can watch Martin demonstrate the technique in his bagel video.) A floured linen is helpful to prevent sticking but a floured or cornmeal-coated sheet will work. (The whole process is actually easier to do than it is to describe. To do it, you hold the ball of dough in your hand and push your fingers through the center from both sides to form a ring, which you then stretch out to bagel dimensions by gently rolling both index fingers around the inside of the hole. The belly button poke technique is both easier to pull off and far gentler on the dough. A gentle stretch creates the right shape. (To achieve even more tension, you can corkscrew the rope around itself a few times before forming the loop.) For a visual of the rope-and-loop, watch Jeffrey Hamelman's episode of the Isolation Baking Show on bagels. Because it involves a lot of dough manipulation, this is the method of choice when you want to build a lot of tension into the dough. You then wrap the rope all the way around your open hand and roll your palm over the overlapping ends to seal them together. In the first method, you form the pre-shaped ball of dough into a rope. There are two basic ways to shape a bagel: the rope-and-loop method, and - as my friend and fellow bagel-baker Jess Wagoner likes to call it - the “belly button poke." A good amount of the chew in a bagel should come from the baker building tension into the dough during shaping. The third element that determines the texture and crumb structure of a bagel is how the bagel is shaped. ![]() But bread flours with a more modest amount of protein (around 12%, like our 12.7% unbleached bread flour) can make an excellent bagel too, albeit one with a more tender, open crumb as can the inclusion of whole grain or high-extraction flours, which provide more flavor and texture as well. New York-style bagels with an intense chew are usually made with high-gluten flour that has a protein percentage at or above 14%. ![]() Flourįlour choice plays a major role in determining a bagel’s texture and crumb structure. Higher-hydration bagels are also easier to mix: Low-hydration bagel doughs can be a challenge to knead fully without some serious effort, even in a stand mixer. Recipes on the higher end of that hydration scale produce a breadier, more open-crumbed bagel, which can be nice they also have the added benefit of being somewhat longer-lasting. It’s also why even the best bagels should be eaten within a few hours of baking - with so little moisture in the dough, the crumb will stale quickly. This helps give them their signature tight crumb structure and their chew. Hydrationīagels are usually made from a stiff, dry dough, with hydrations in the range of 55 to 65% (compared to soft sandwich or crusty artisan breads, which are usually 65% hydration and higher). Bagel dough is stiffer than many bread doughs.
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This is also backed up by the fact that I didn't have a high percentage of dead cells or low number of total cells for the conditions in which there was no necrotic spleen tissue (negative controls and Ova+CpG). I suspect that I may have lost a lot of my live cells when I removed this pellet, as my collaborator mentioned that often the live cells will stick to the dead cells. I did notice that there was an insoluble pellet of dead spleen tissue that I had to remove from the tubes in most of the experimental conditions post-ACK lysis. We then did an ACK lysis step to remove RBCs, after which we counted the cells. These 5 organs were placed onto a 40uM filter and mashed up with the rubber end of a 1mL syringe, followed by 4 successive washes with media (we pooled the lymphocytes and splenocytes together, again to try and maximize our chances of seeing our Ova specific T cells). Does anybody know how long after a booster injection I should look at the T cells?įor obtaining lymphocytes and splenocytes, we removed spleens and both inguinal and popliteal lymph nodes from each mouse. Is it possible that I waited too long after the booster injection to analyze the T cells? What I'm thinking is that by 7 days after the boost, most of my Ag-specific T cell population has died off, perhaps due to lack of persistent antigenic stimulation. Additionally, gating on the remaining dead cell population in FlowJo revealed that these cells were primarily CD8+ Tetramer+. ![]() I believe I lost a lot of my Ag-specific T cells in this dead cell population - mostly due to the fact that despite the macroscopic evidence of an immune response I wasn't able to see an increased percentage of antigen specific T cells. The lymph nodes in the experimental conditions were quite enlarged and inflamed however, there was also a huge amount of cell death in these conditions. I analyzed the Ova-specific splenocytes and lymphocytes on D14 with MHC tetramers. The regimen is a primary injection on D0 followed by a booster injection on D7. I'm performing an In vivo experiment in which I inject mice with a DC vaccine containing ovalbumin. It's also a question of who will use the software in the lab - if it is only you and you are already a FlowJo expert you may stick with it, but if there are people less adept with it I think Cytof is easier to learn, and since there is less flexibility analyses from other users are easier to understand and to review. But if you need something different, there is no way to change it. On the other hand, Flowjo also has some features which at least I have not found yet in Cytobank (overlay of scatterplots for example), and it allows for more fine tuning, customizing and parameters to be defined by the user (like setting gates for compensation manually etc.), whereas Cytobank sometimes appears a bit like an Apple product - very good, very intelligent, perfect for 90% of all situations. ![]() So, when you do Cytof or more than 15 channel FACS and you want to use tools like Spade or Visne there is probably no way around it. ![]() Cytobank has very nice and modern looking graphs, a very intuitive and intelligent work flow and user-interface and is very good with high-throughput and especially multi-channel analyses. In my opinion it depends a lot on what you plan to do with it and what kind of "style" you prefer. ![]() (I can’t recommend Wiretap as Wiretap users were left in the dark until 2013 regarding 10.7+ support). Hijack makes the list as Rogue Amoeba has kept it up-to-date and it costs less than half the price of Wiretap. To reiterate the above: Audio Hijack Pro allows you to record any audio on your Mac, be it a movie, VoIP chat, radio, web browser, game or otherwise, and export it to AIFF, AAC, MP3, Apple Lossless etc.Īudio Hijack Pro isn’t the first app of its type and it borrows heavily from Wiretap (another Mac only app with similar functionality). Record from microphones, Radiosharks and other hardware. Record from applications like iTunes, Skype or DVD Player. Record any audio - three simple words to explain Audio Hijack Pro. It also exports to the web as interactive presentations. Serious projects call for serious tools. It sports smart guides, widget creation, stylizing, collaboration tools and more. Its one of the few prototyping apps that boasts responsive wireframes, specifically designed for UI Designers. Design beautiful interfaces, invent new widgets, style them, and put it all together in an interactive prototype!Īntetype is unusual as its designed for prototyping websites, apps and even manufacturing projects. AntetypeĪntetype UI designer is a brand new, ground-breaking design application that supports user-interface designers at every step of their work. It works much better than pasting a wad of text in your browser bar and copying it back. The killer feature is one of the simplest which allows you to take any copied text and paste to plain text, stripping any unwanted formatting. It’s actually a utility toolset to make you more productive. Alfred is the ultimate productivity tool for your MacĪlfred markets itself as a productivity app, yet it doesn’t track your time or progress. ![]() Loads of app-launching, file-searching goodness - free for you to download and use with no strings attached. Be more productive with hotkeys, keywords, and file actions at your fingertips. Alfred saves you time when you search for files online or on your Mac. AlfredĪlfred is an award-winning productivity application for OS X. Its perfect for quick photo manipulation. Acorn’s new non-destructive filters and bezier tools, an interface that is reminiscent of the best of both Photoshop and iPhoto makes for a compelling Photoshop alternative.Īcorn is aimed at the prosumer but makes image editing a breeze, even for non-designers. Photoshop might have its place but its price and feature set may be overkill or unneeded. Work with layers to touch up your favorite photos or make something new from scratch. Combine images together to create your own. Add text and shapes to your digital pictures. “Everyone needs to edit images at some point, but not everyone has the time to learn complicated super pricey image editing programs. You probably don’t need every single one of these apps or utilities, but they all are well worth purchasing or downloading. I’ve labored over this list, trying to get the best of the best and spent more time than I care to admit, compiling it. If I missed any, feel free to let me know.Ĥ4 Mac only Design / Development Utilities and Apps Acorn (Black Pixel, Bohemian Coding, Mac Rabbit, Panic Studios, Pixel Cut and Rogue Amoeba). Some of the applications in this list are redundant (ex: BBedit, Coda, Espresso), a few defy simply graphic design / web design (BetterTouchTool, Crossover, djay, Little Snitch, Parallels Desktop, Playback, Radium) and yet a few more aren't intrinsically aimed at professionals (Acorn, Permute and Radium). Reflect great design/usability and usefulness.One that consisted of the app name, icon, a marketing blurb from the authors and my own personal take. These applications are often built with a different mindset, one that embraces the Apple aesthetic of interface design. I decided to compile a list of these apps, without silly rankings or any obligation prune the list to fit a numerical objective Instead, I opted for a simple list. Some of these tools are relatively young and some have been around over two decades There’s plenty of cross platform solutions made by big players but the most avant-garde and forward thinking tools are coming from small studios. 53 Mac Only Design/Development Apps (Oct 2013) ![]() Sure, you could opt to start your design sketches on lined paper if you really wanted to, but you absolutely need a drawing pad. It’s time to acquire your fashion canvas. Although we wish we could draw the perfect design, reality (and further inspiration) gets in the way. You could also use charcoal for blending or ink for a distinct, striking look. Settle on a brand of drawing pencil you like and start to experiment with your designs. These are the primary tools to make your sketch come to life on the factory floor. ![]() What You Need For a Fashion Sketchīefore launching into a creative fashion sketch, it’s useful for any designer to compile the following. Overall, this is one of the essential steps in garment manufacturing. There are different types of sketches and drawings that a designer can produce.īut first, they need a few key tools to make a drawing worthy of a future fashion design. Even kids are getting in on the benefits of being talented illustrators.īut first, what is a fashion sketch? What Is A Fashion Sketch?Ī fashion sketch is a detailed drawing where the fashion designer envisions their design on a head or body. Let’s see how to create a fashion drawing. ![]() It’s a great skill to hone for any future fashion designs that pop into your head. Namely, knowing how to fashion sketch is one of the building blocks to any good clothing line.Īnd, if you’ve landed on the right clothing manufacturer, you could receive some invaluable assistance. On the exciting journey towards starting a successful clothing line, numerous moving parts make it memorable and unique. ![]() Granted, it’s a large sum of money, but he also puts in the time. CNN currently pays him approximately $5 million per year. How exactly has he made it all? As discussed at the beginning of this article, it all comes down to hard work. That’s a considerable amount of money, to be sure. Wolf Blitzer Net WorthĪs previously mentioned, Blitzer currently has a net worth of $20 million. That in turn has helped him achieve his net worth of $20 million. This has allowed him to command one of the highest salaries in journalism. As a result, he has become indispensable at the network. He puts countless numbers of hours in behind the scenes, doing research and uncovering even the slightest angle, things that are often overlooked by other people. For him, it’s not just about getting in front of a camera and telling a story. If the story is even remotely political in nature, you can bet that Blitzer is at the heart of it. In addition, he has long been the primary political correspondent for the network. Eventually, he was even offered his own show, something that only a handful of individuals ever achieve. He had achieved elite status at one of the world’s most prestigious news organizations. The Later Yearsīy the year 2000, Blitzer had done the one thing that so many up-and-coming journalists hope to accomplish. He was quickly moving up the ranks as a millionaire, making an impressive name for himself in the process. Wolf Blitzer had made it his home and he clearly knew what to do. At the time, it was easily one of the most well-respected news networks out there.Īs a result, most people considered it an honor just to have a chance to work there. It’s also worth noting that this was largely during CNN’s hayday, when the network was in its prime. Blitzer was quickly becoming the reporter that CNN leaned on every time they wanted to be first out of the gate with something. Once again, his work there was outstanding, garnering him even more accolades. When the Oklahoma City bombing happened in 1995, they sent him to the site of the crime and allowed him to do his job the best way he knew how. More AcclaimĪfter his outstanding work covering the Gulf War, CNN wanted him to be on the scene for every major news story. This landed him a hefty promotion and a better salary to go with it, all by the mid-1990s. In fact, it was largely because of him that the entire network won an award for their coverage of the Gulf War. He didn’t mind coming in early and staying late in order to make sure that the piece he was working on was impeccably handled. His work ethic was practically unmatched. He was always willing to go where others weren’t, even if it meant traveling directly into the epicenter of the fighting. In fact, he started working there roughly around the same time that the Gulf War broke out, something which he covered in-depth. As far as his career at CNN is concerned, he’s been a constant presence there since 1990. After that, he moved directly into the field of journalism and that is where he has been ever since. He attended college in New York state in the early 1970s, obtaining first his bachelor’s degree and then his master’s. Wolf Blitzer has been working in journalism for a very long time. Once you’ve unlocked that disk, click on the button to set startup security for that disk. You’ll then be prompted to select the system for which you want to configure startup security. Once the main window has loaded, select the Startup Security Utility in the Utilities menu. Select the Options icon, then click Continue underneath it. This takes you to the Startup Options screen. If you want your M1 Mac to load a new kext, you’ll need to start it up in Recovery Mode, with the Power button held until the display shows Loading Startup Options, then release it. Strictest rules come with M1 Macs: they have a new platform security setting which blocks the loading of all third-party kexts, and that’s turned on by default. However, given that kexts run in kernel space, they have access to the very heart of the system, so Apple has progressively tightened security measures imposed on them. What used to happen was that third-party kexts were installed in the folder /Library/Extensions, incorporated into a new prebuilt kernel, and were quickly ready for use without any further fuss. This article explains what happens, and what you’ll need to do. This may not be true of some free software, and in any case the process requires your involvement. Normally, when you install third party products which rely on extensions, they should also install any necessary extensions for you. ![]() Although developers are moving their old kexts to the new varieties, this isn’t an easy task, so please bear with them as they do. kext and run in kernel space, and modern system extensions and their relatives which run in user space. There are two main types of extension in macOS: traditional kernel extensions, which have the extension.
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![]() Que 1 – How can I import Kerio contacts in Mozilla Thunderbird?Īns – You can follow these steps to migrate contacts from Kerio to Thunderbird. ![]() Now press the Next option to continue.Ĩ – Kerio connect to Thunderbird migration process is running.ĩ – Kerio emails to Thunderbird migration procedure has been finished successfully.ġ0 – At last, run Mozilla Thunderbird email client to access imported Kerio profile data. Desktop or Laptop.Ģ – Start the tool and choose one option from Select Files or Select Folders for uploading Kerio profile data.ģ – Check I am converting Multiple Users option if required and after that choose Select Folder… button.Ĥ – Browse Kerio profile location from the default storage location and hit on the Next button.ĥ – Select Kerio connect folders from software GUI and click on the Next button to continue.Ħ – Choose Thunderbird from 30+ saving formats.ħ – After that, Kerio to Thunderbird Converter automatically chooses default storage location to store output data. Move Emails from Kerio to Thunderbird – Step-by-Stepġ – Download Kerio to Thunderbird Converter and install it on your Windows Computer i.e.
![]() Youngest son Alan attends a boarding school in Massachusetts, with his best friend Fermat Hackenbacker, son of the Thunderbirds’ resident engineer Brains, and dreams of being a Thunderbird pilot. The Tracy family, led by former astronaut Jeff Tracy, operate International Rescue (IR), a secret organization that aids those in need during disasters using technologically advanced machines called Thunderbirds, operating out of Tracy Island in the South Pacific. The film's soundtrack includes the songs " Thunderbirds Are Go" by pop-rock band Busted, which peaked at number one in the UK charts and later won the 2004 UK Record of the Year award and Take Me Away by Caleigh Peters which was used for other countries excluding the UK. Gerry Anderson was also critical of the film, describing it as "the biggest load of crap I have ever seen in my entire life", although Sylvia Anderson praised it as a "great tribute" to the series. ![]() The film received negative reviews from critics, who disparaged its wooden characters and thin plot, and was a box-office bomb. In contrast with the original TV series, which used a combination of puppetry and scale-model special effects dubbed " Supermarionation", this film was made in live action with CGI effects. The film's plot concerns the Hood, who traps International Rescue (IR) leader Jeff Tracy and four of his sons on board the damaged Thunderbird 5 to steal the other Thunderbirds vehicles and commit heists that IR will be blamed for, prompting Jeff's youngest son Alan and his friends Tin-Tin and Fermat to stop him. The film, written by William Osborne and Michael McCullers, was released on 20 July 2004 in the United Kingdom and 30 July 2004 in the United States. The selling dealer states that the generator, transmission mounts, and various gaskets and seals were replaced in July 2022.Īdditional underbody photographs are provided in the gallery below.Thunderbirds is a 2004 science fiction action- adventure film directed by Jonathan Frakes, based on the 1960s TV series Thunderbirds created by Gerry and Sylvia Anderson. The 352ci V8 is equipped with a single carburetor as well as “Powered by Ford”-logo valve covers, and it routes power to the rear wheels through a three-speed Cruise-O-Matic automatic transmission. The five-digit mechanical odometer shows 12k miles. Instrumentation includes a 140-mph speedometer flanked by a clock, switches, and auxiliary gauges. The three-spoke steering wheel is equipped with a horn ring and wears an aftermarket wrap. Equipment includes a center console, a MagicAire heater/defroster, power windows, and lap belts. The front bucket seats and the rear bench seat are upholstered in red and white vinyl, and matching upholstery covers the door panels. The car is equipped with power-assisted steering, and the selling dealer states that the power-assisted drum brakes were serviced with replacement shoes, cylinders, and hoses at each corner. ![]() Steel 14″ wheels wear bright covers and are wrapped in 215/75 Goodyear Polysteel Radial thin-whitewall tires. The winner has been banned from future participation on BaT and their buyer’s fee was not refunded. This was not due to any fault with the vehicle. This is a re-list after the vehicle was previously sold on BaT in January 2023 and the winning bidder failed to follow through with their commitment to purchase. The selling dealer notes areas of corrosion below the fenders and on the underside. The car was reportedly fitted with replacement weatherstripping and door seals in July 2022. Exterior features include a hood scoop, fender skirts, chrome bumpers, rear quarter-panel fins, and quadruple headlights and taillights. The car left the factory finished in Colonial White and is said to have been repainted under previous ownership. This Thunderbird is offered on dealer consignment at no reserve in Texas with a clean Colorado title. Work performed in July 2022 is said to have included replacing the generator, battery, transmission mounts, braking components, weatherstripping, and various gaskets and seals. Equipment includes a center console, a MagicAire heater, and 14″ steel wheels with bright covers as well as power steering, brakes, and windows. Power comes from a 352ci V8 paired with a Ford Cruise-O-Matic three-speed automatic transmission, and the car is finished in white over two-tone vinyl upholstery. This 1959 Ford Thunderbird was reportedly refurbished before the current owner’s acquisition in 2022, and it was previously listed on BaT in December 2022. |